Ka hoʻomohala ʻana i kahi ala ELISA indirect no ka ʻike ʻana i ka porcine acute diarrhea syndrome coronavirus IgG antibody e pili ana i ka protein spike recombinant.

ʻO ka Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) kahi mea hou i ʻike ʻia he porcine enteric pathogenic coronavirus e hiki ai ke hoʻoulu i ka maʻi maʻi wai i nā puaʻa hānau hou a hoʻoulu i nā poho waiwai nui i ka ʻoihana puaʻa.I kēia manawa, ʻaʻohe ala serological kūpono e loiloi i ka maikaʻi o ka maʻi SADS-CoV a me ke kano, no laila, pono e hoʻohana i kahi enzyme-linked immunosorbent assay (ELISA) kūpono e hoʻoponopono ai i kēia hemahema.Ma ʻaneʻi, ua kūkulu ʻia kahi plasmid recombinant e hōʻike ana i ka protein SADS-CoV spike (S) i hui pū ʻia me ka domain IgG Fc kanaka e hana i ka baculovirus recombinant a hōʻike ʻia i nā cell HEK 293F.Hoʻomaʻemaʻe ʻia ka protein S-Fc me ka protein G resin a paʻa i ka reactivity me nā anti-human Fc a me anti-SADS-CoV antibodies.A laila ua hoʻohana ʻia ka protein S-Fc e hoʻomohala i ka ELISA indirect (S-iELISA) a hoʻopaʻa i nā ʻano hopena o S-iELISA.ʻO ka hopena, ma ka nānā ʻana i ka waiwai OD450nm o 40 SADS-CoV negative sera i hoʻopaʻa ʻia e ka immunofluorescence assay (IFA) a me Western blotting, ua hoʻoholo ʻia ka waiwai ʻoki ʻia he 0.3711.ʻO ka coefficient of variation (CV) o ka 6 SADS-CoV positive sera i loko a ma waena o nā holo o S-iELISA i emi iho ma mua o 10%.Ua hōʻike ʻia ka hoʻāʻo cross-reactivity ʻaʻohe S-iELISA ʻaʻohe cross-reactivity me nā sera virus porcine ʻē aʻe.Eia kekahi, ma muli o ka ʻike ʻana o 111 mau hōʻailona serum clinical, ʻo ka nui o ka coincidence rate o IFA a me S-iELISA he 97.3%.Ua hōʻike ʻia ka hoʻāʻo neutralization virus me 7 mau waiwai OD450nm like ʻole o ka serum i ka waiwai OD450nm i ʻike ʻia e S-iELISA i hoʻopili maikaʻi ʻia me ka hoʻāʻo neutralization virus.ʻO ka hope, ua hana ʻia ʻo S-iELISA ma 300 puaʻa puaʻa serum samples.Ua hōʻike ʻia nā pahu pāʻoihana o nā enterovirus porcine ʻē aʻe he 81.7%, 54%, a me 65.3% ka nui o ka IgG maikaʻi o SADS-CoV, TGEV, PDCoV a me PEDV., 6%, kēlā me kēia.Hōʻike nā hopena i ka S-iELISA he kikoʻī, paʻakikī, a hiki ke hoʻohana hou ʻia, a hiki ke hoʻohana ʻia e ʻike i ka maʻi SADS-CoV i ka ʻoihana puaʻa.Mālama ʻia kēia ʻatikala e ke kuleana kope.Ua mālama ʻia nā pono āpau.